chapter 05

Biolo1100 Chapter 5 DNA, Gene Expression, and Biotechnology

DNA is a double helix macromolecule made of nucleotides .
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Sequences of nucleotides called genes control cellular functions and make up an individual's genome.
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Much of the human genome are non-coding DNA that are not genes .
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Gene expression (exhibiting a phenotype) involves 2 steps:
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- Transcription involves copying genetic information on DNA to a RNA transcript.
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- Translation involves the production of proteins from nucleotide sequences copied onto the RNA transcript.
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A mutation is a change in the DNA sequence that may affect proteins.
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Genetic engineering technologies have introduced genetically modified organisms that provide new opportunities and risks.
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Mammals can be cloned by nuclear transfer.
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DNA fingerprinting makes use of several genetic technologies.
- Short tandem repeats provide genetic markers.
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- Gel electrophoresis separates DNA fragments.
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- DNA fingerprints can identify individuals based on their DNA.
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